Immunological determination of faecal haemoglobin concentrations in dogs

Faecal haemoglobin (Hb) concentrations in apparently healthy experimental Beagle dogs and in dogs of various breeds kept in private households or at breeders were measured by reversed passive latex agglutination (RPLA) and enzyme-linked immunosorbent assay (ELISA) in an effort to define the physiological concentrations of faecal Hb in the dog. In 88% (53) of 60 experimental Beagle dogs (30 males and 30 females), the RPLA titres were 1:2 and 1:8 and the faecal Hb concentrations ranged from 40.0 to 431.5 (mean 184.1±92.6) g/g faeces by ELISA. No significant difference was found in Hb levels or RPLA titres between males and females. Seven dogs (12%) had significantly greater RPLA titres and Hb concentrations by ELISA than the remaining dogs. In 84% (45) of the 53 dogs kept in private households or at breeders, the RPLA titres were >1:1 to 1:8 and the faecal Hb concentrations ranged from 7.1 to 456.7 (mean 137.5±128.7) g/g faeces in ELISA. Eight of these dogs (15.1% of 53 dogs) had significantly greater RPLA titres and Hb concentrations by ELISA than the remaining dogs. There were no significant differences between the Beagles and dogs kept in private households or at breeders. In conclusion, in 98 (86.7% of 113) dogs the physiological concentrations of RPLA titres were >1:1 to 1:8 and the faecal Hb concentrations were 143.5–185.1 g/g (95% confidence level). Approximately 13.3% of apparently healthy dogs had higher faecal Hb concentrations, suggesting the presence of subclinical haemorrhages. Four dogs suffering from colorectal cancer also had high faecal Hb concentrations.     

大麦日粮中添加粗酶制剂对鸡外周血液中代谢激素的影响

为探讨粗酶制剂促进鸡生长的作用机制,通过三系列试验研究了粗酶制剂添加于大麦日粮中对不同基因型或生理状况的鸡血液代谢激素的影响。结果表明,粗酶制剂处理,使蛋用雏鸡Ｔ３（三碘甲腺原氨酸）、ＴＳＨ（促甲状腺激素）和Ｔ３／Ｔ４（甲状腺素）显著提高（Ｐ＜０．０１或Ｐ＜０．０５）;２１日龄肉鸡Ｔ３、Ｉｎｓ（胰岛素）、Ｔ３／Ｔ４和Ｉｎｓ／Ｇｌｕｃ（胰高血糖素）提高（Ｐ＜０．０５）;４２日龄肉鸡Ｔ３、ＧＨ（生长激素）、Ｉｎｓ、Ｔ３／Ｔ４和Ｉｎｓ／Ｇｌｕｃ显著提高（Ｐ＜０．０５）,而Ｇｌｕｃ显著降低（Ｐ＜０．０５）;生长后期鸡Ｔ３、ＧＨ和Ｔ３／Ｔ４显著升高（Ｐ＜０．０５）。结果提示,粗酶制剂可通过调节代谢激素的分泌而提高鸡的生产性能。     

Immunophenotypic comparison of blood and lymph node from dogs with lymphoma

Peripheral blood and lymph node tissue from 12 dogs with lymphoma was immunophenotyped. Additionally, the bone marrow was immunophenotyped in 6 dogs. The lymphomas were characterized as B-cell in 11 dogs and T-cell in 1 dog. Immunophenotypic patterns in the peripheral blood and bone marrow were variable. The trend in dogs with B-cell lymphoma was normal to increased percentage of IgG-positive cells, decreased percentage of pan-T-positive cells, decreased percentage of CD4-positive cells, and decreased CD4/CD8 ratio. Simultaneous immunophenotyping of lymph node, blood and bone marrow cannot be recommended routinely without further studies to document its value as an independent prognostic indicator. However, it is potentially useful for tumor staging and monitoring remission, especially in lymphoma patients with a leukemic phase.     

雏鸡感染传染性法氏囊病病毒时血细胞动态变化

研究了感染ＩＢＤＶ免疫和未免疫３０～５９日龄ＡＡ雏鸡的外周血细胞动态变化。结果表明：ＩＢＤＶ对红细胞数（ＲＢＣ）影响各组差异不显著,均随日龄增长呈上升趋势;从以ＩＢＤＶ攻毒前１ｄ到攻毒后的头５ｄ,３组鸡的白细胞数（ＷＢＣ）均呈上升趋势,未免疫未攻毒鸡（Ｃ组）随后下降,而未免疫攻毒鸡（Ａ组）和免疫攻毒鸡（Ｂ组）则持续上升至攻毒后第７ｄ,然后下降;Ａ组在ＩＢＤＶ攻毒后,白细胞分类计数（ＤＣ）中的单核细胞和异嗜性粒细胞急剧增加,淋巴细胞第１ｄ虽有减少但随后上升,嗜碱性粒细胞和嗜酸性粒细胞第７ｄ出现峰值,以后下降。方差分析显示,Ａ组ＷＢＣ和ＤＣ均高于或显著高于Ｂ组和Ｃ组,Ｂ、Ｃ两组比较,差异不明显。     

睾丸摘除对水牛血像和血液免疫功能指标的影响

选择６头健康、２～３岁、体重３００～４００ｋｇ雄性水牛,研究了睾丸摘除对血像和某些免疫功能指标的影响。结果表明：睾丸摘除后ＲＢＣ、ＰＣＶ、Ｈｂ、ＲＣＣ虽与摘除前比较有不同程度的变化,但差异均不显著;ＷＢＣ在睾丸摘除后１５ｄ内呈明显下降趋势,ＤＣ中的嗜酸性粒细胞、淋巴细胞和单核细胞（％）在摘除后１～４ｄ内降低或显著降低,而嗜中性粒细胞则显著升高,嗜碱性粒细胞摘除前后无明显变化;ＲＢＣ－ＣＲ１花环率和Ｂ－淋巴细胞（％）在摘除后１５ｄ内明显低于摘除前水平,ＲＢＣ－ＩＣ花环率在摘除后第５天明显下降,以后又逐渐回升到摘除前水平,Ｔ－淋巴细胞变化与Ｂ－淋巴细胞相反;血清总蛋白（ＴＰ）、白蛋白（Ａ）分别在摘除２ｄ后和头２ｄ显著下降,以后ＴＰ在低水平上变动,而Ａ略有回升,球蛋白（Ｇ）、β－Ｇ和γ－Ｇ在摘除后头２ｄ内均明显升高,以后变化与摘除前水平比较差异不显著,α－Ｇ在摘除前后无明显变化。     

Effect of cholecystokinin-octapeptide on focal cerebral ischemia/reperfusion injury in rats

AIM:To examine the effect of cholecystokinin-octapeptide (CCK-8) on focal cerebral ischemia/reperfusion injury and its underlying mechanisms.METHODS:By using the suture model of focal cerebral ischemia and reperfusion, the effects of intracerebroventricular (icv) injection of CCK-8 and proglumide, nonselective CCK receptors antagonist, on the infarct size, regional cerebral blood flow (rCBF), and the levels of nitric oxide (NO), malondialdehyde (MDA) were observed in different brain regions of rats subjected to 1 h focal cerebral ischemia followed by 24 h reperfusion.RESULTS:(1) pretreatment with different doses of CCK-8 (0.3 μg,1.0 μg,2.0 μg or 4.0 μg) could attenuate the infarct size, but the statistically significant effects of CCK-8 were obtained only at the doses of 1.0 μg and 2.0 μg(P＜0.05). The neuroprotective effects of CCK-8 were blocked by pretreatment with proglumide. Administration of proglumide alone could worsen the ischemia/reperfusion injury. (2) CCK-8 (1.0 μg) inhibited the increase in NO, MDA levels in the ischemic core, and also inhibited the increase in NO level in the ischemic penumbra. The rCBF in the CCK-8 group was significantly higher than the normal value at 24 h after reperfusion (P＜0.05).CONCLUSIONS:These results suggest that both endogenous and exogenous CCK-8 alleviate focal cerebral ischemia/reperfusion injury. Such an action may be associated with inhibition of free radical-induced injuries and the improvement in rCBF.     

Changes of regional cerebral blood flow in AIDS patients without acquired immunodeficiency syndromes dementia complex

AIM:To investigate the changes of regional cerebral blood flow(rCBF) in AIDS patients without acquired immunodeficiency syndrome dementia complex(ADC).METHODS:^99mTc-ECD brain SPECT imaging was performed in 4 AIDS patients without ADC and 16 sex and age-matched normal controls. The rCBF percentage compared to the cerebellum were calculated using a semi-quantitative processing software.RESULTS:The rCBF in the right and left frontal, temporal, porietal lobe, basal ganglia and thalamus, cyrus rectus and pons were decreased significantly in AIDS patients without ADC than those of the control subjects (P＜0.01).CONCLUSION:There is reduced cortico-subcortical rCBF in AIDS patients without ADC.     

绿洲规模扩张的阀限与预警指标体系框架建构

本文讨论了绿洲规模扩张的阀限 ,认为干旱区绿洲扩张要受到资源、环境与生态的限制 ,绿洲规模一旦超过这些阀限 ,将会出现严重的人地关系失调。在此基础上探讨了绿洲规模扩张预警的意义以及指标体系建构的原则与步骤 ,提出了绿洲规模扩张的预警指标体系     

四个不同节气对哈巴特肉仔鸡血糖值的影响

[摘要]为比较哈巴特肉仔鸡在春分、夏至、秋分和冬至四个不同节气时的血糖值,分别在同一年度的春分、夏至、秋分和冬至的当天测定12日龄哈巴特肉仔鸡的空腹血糖值。结果表明：哈巴特肉仔鸡的血糖值在夏至时为最低,冬至时为最高,且冬至时极显著地高于春分和夏至（P〈0．01）,秋分时极显著地高于夏至（P〈0．01）,而血糖值春分和秋分之间无显著性差异（P〉0．05）。可见,随着季节的变换,哈巴特肉仔鸡的血糖值也发生相应变化。     

谷氨酰胺、壳聚糖及二者复合添加对断奶仔猪生长性能和血液生化指标的影响

选取192头体重相近(平均体重为5.27kg)的(21±2)日龄PIC断奶仔猪,按胎次、公母分4个处理组,每组3个重复,每个重复16头。试验分两个阶段:21～35日龄、35～49日龄,研究谷氨酰胺、壳聚糖单独和复合添加对断奶仔猪生产性能和血液生化指标的影响。结果表明:①日粮中单独添加1%谷氨酰胺或0.04%壳聚糖有提高断奶仔猪日增重、降低料重比的趋势。②谷氨酰胺与壳聚糖复合添加使用在一些指标上优于单独添加,体现出一定的协同作用。